Journal Article

Identification of the Region Required for Monomerization of the Rolling Circle Plasmid pKYM

Hiroo Yasukawa, Eric Viller and Yukito Masamune

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 5, issue 1, pages 11-14
Published in print January 1998 | ISSN: 1340-2838
Published online January 1998 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/5.1.11

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Plasmid pKYM, isolated from the Gram-negative bacterium Shigella sonnei, is a small multicopy plasmid which replicates by a rolling circle mechanism. The formation of multimers has been observed in a derivative of pKYM which lost a part of the origin region, and the loss of the monomerization mechanism would have led to these multimers. By analyzing the constructs of several mutants, we discovered that a DNA region required for monomerization was present upstream of the RepK binding site in the replication origin. As either of the T-rich sequence or the inverted repeat sequences which were seen in that region have been lost in the multimer-forming plasmids, these sequences may be necessary for monomerization.

Keywords: rolling circle; pKYM; monomerization; DNA replication

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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