Journal Article

Structural Analysis of <i>Arabidopsis thaliana</i> Chromosome 5. V. Sequence Features of the Regions of 1,381,565 bp Covered by twenty one Physically assigned P1 and TAC Clones

Takakazu Kaneko, Hirokazu Kotani, Yasukazu Nakamura, Shusei Sato, Erika Asamizu, Nobuyuki Miyajima and Satoshi Tabata

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 5, issue 2, pages 131-145
Published in print January 1998 | ISSN: 1340-2838
Published online January 1998 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/5.2.131

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The nucleotide sequences of 21 Pl and TAC clones which have been precisely localized to the fine physical map of the Arabidopsis thaliana chromosome 5, were determined, and their sequence features were analyzed. The total length of the regions sequenced in this study were 1,381,565 bp, bringing the total length of the chromosome 5 sequences determined so far to 6,691,670 bp together with the regions of the 69 clones previously reported. By computer-aided analyses including similarity search against protein and EST databases and gene modeling with computer programs, a total of 337 potential protein-coding genes and/or gene segments were identified on the basis of similarity to the reported gene sequences. An average density of the genes and/or gene segments thus assigned was 1 gene / 4,100 bp. Introns were identified in 76.7% of the potential protein genes for which the entire gene structure were predicted, and the average number per gene and the average length of the introns were 3.9 and 176 bp, respectively. These sequence features are essentially identical to those in the previously reported sequences. The numbers of the Arabidopsis ESTs matched to each of the predicted genes have been counted to monitor the transcription level. The sequence data and gene information are available on the World Wide Web database KAOS (Kazusa Arabidopsis data Opening Site) at .

Keywords: Arabidopsis thaliana chromosome 5; genomic sequence; P1 genomic library; TAC genomic library; gene prediction

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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