Journal Article

A Physical Map of <i>Arabidopsis thaliana</i> Chromosome 3 Represented by Two Contigs of CIC YAC, P1, TAC and BAC Clones

Shusei Sato, Hirokazu Kotani, Reiko Hayashi, Yao-Guang Liu, Daisuke Shibata and Satoshi Tabata

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 5, issue 3, pages 163-168
Published in print January 1998 | ISSN: 1340-2838
Published online January 1998 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/5.3.163

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We have constructed a physical map of Arabidopsis thaliana chromosome 3 by ordering the clones from CIC YAC, P1, TAC and BAC libraries using the sequences of a variety of genetic and EST markers and terminal sequences of clones. The markers used were 112 DNA markers, 145 YAC end sequences, and 156 end sequences of P1, TAC and BAC clones. The entire genome of chromosome 3, except for the centromeric and telomeric regions, was covered by two large contigs, 13.6 Mb and 9.2 Mb long. This physical map will facilitate map-based cloning experiments as well as genome sequencing of chromosome 3. The map and end sequence information are available on the KAOS (Kazusa Arabidopsis data Opening Site) web site at .

Keywords: Arabidopsis thaliana; chromosome 3; physical map; contig map

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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