Journal Article

Clustering of Human Chromosome Fragments on the Mouse Genome by Chromosome-Mediated Gene Transfer

Hideki Ogino, Kazuhiko Nakabayashi, Eriko Michishita, Stephen W. Scherer, Michihiko Fujii, Toshikazu Suzuki and Dai Ayusawa

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 5, issue 4, pages 247-250
Published in print January 1998 | ISSN: 1340-2838
Published online January 1998 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/5.4.247

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Pieces of metaphase chromosomes prepared from mouse cells containing neo-tagged human chromosome 7 were transferred to mouse cells with calcium phosphate to isolate G418-resistant clones. FISH analysis revealed that the majority of them contained human DNA at a single site on their genome. These transformants contained STS markers mapped to various regions of chromosome 7. It is thus suggested that pieces of human chromosomes tend to assemble and integrate on the mouse genome.

Keywords: chromosome transfer; chromosome 7; Alu-PCR; STS marker

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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