Journal Article

Phosphate Transporter Gene Family of <i>Arabidopsis thaliana</i>

Satoru Okumura, Norihiro Mitsukawa, Yumiko Shirano and Daisuke Shibata

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 5, issue 5, pages 261-269
Published in print January 1998 | ISSN: 1340-2838
Published online January 1998 | e-ISSN: 1756-1663 | DOI:

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Using a high-affnity phosphate transporter gene of Arabidopsis thaliana, PHT1, as a probe, three Arabidopsis homologs were isolated, named PHT4, PHT5 and PHT6, in addition to the previously isolated PHT2 and PHT3. The amino acid sequences deduced from the three nucleotides were 32–42% homologous with microbial phosphate transporters of Saccharomyces cerevisiae (PHO84), Neurospora crassa (PHO-5) and Glomus versiforme (GvPT). PHT1, PHT2, PHT3 and PHT6 were clustered in a 25-kbp region of chromosome V. PHT1 and PHT4 transcripts were detected in roots. Interestingly, suspension-cultured cells expressed only PHT4. PHT4 and PHT5 located within a genetic distance of 6.4 cM on chromosome II, and were close to a phosphate accumulation mutant pho2. Genomic sequencing revealed no difference in the sequences of the two genes in both pho2 and wild-type. The PHT4 transcript was expressed at similar levels in the mutant and wild-type. These results demonstrate that neither PHT4 nor PHT5 is allelic to PHO2.

Keywords: Arabidopsis thaliana; phosphate transporter; PHO84; pho1; pho2

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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