Journal Article

The <i>Dictyostelium</i> Developmental cDNA Project: Generation and Analysis of Expressed Sequence Tags from the First-Finger Stage of Development

Takahiro Morio, Hideko Urushihara, Tamao Saito, Yoshihiro Ugawa, Hideaki Mizuno, Motonobu Yoshida, Ryuji Yoshino, Biswa Nath Mitra, Min Pi, Tomihiro Sato, Keiko Takemoto, Hiroo Yasukawa, Jeffrey Williams, Mineko Maeda, Ikuo Takeuchi, Hiroshi Ochiai and Yoshimasa Tanaka

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 5, issue 6, pages 335-340
Published in print January 1998 | ISSN: 1340-2838
Published online January 1998 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/5.6.335

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In an effort to identify and characterize genes expressed during multicellular development in Dictyostelium, we have undertaken a cDNA sequencing project. Using size-fractionated subsets of cDNA from the first finger stage, two sets of gridded libraries were constructed for cDNA sequencing. One, library S, consisting of 9984 clones, carries relatively short inserts, and the other, library L, which consists of 8448 clones, has longer inserts. We sequenced all the selected clones in library S from their 3′-ends, and this generated 3093 non-redundant, expressed sequence tags (ESTs). Among them, 246 ESTs hit known Dictyostelium genes and 910 showed significant similarity to genes of Dictyostelium and other organisms. For library L, 1132 clones were randomly sequenced and 471 non-redundant ESTs were obtained. In combination, the ESTs from the two libraries represent approximately 40% of genes expressed in late development, assuming that the non-redundant ESTs correspond to independent genes. They will provide a useful resource for investigating the genetic networks that regulate multicellular development of this organism.

Keywords: Dictyostelium discoideum; cDNA project; ESTs; multicellular development

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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