Journal Article

Prediction of the Coding Sequences of Unidentified Human Genes. XII. The Complete Sequences of 100 New cDNA Clones from Brain Which Code for Large Proteins <i>in vitro</i>

Takahiro Nagase, Ken-ichi Ishikawa, Mikita Suyama, Reiko Kikuno, Makoto Hirosawa, Nobuyuki Miyajima, Ayako Tanaka, Hirokazu Kotani, Nobuo Nomura and Osamu Ohara

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 5, issue 6, pages 355-364
Published in print January 1998 | ISSN: 1340-2838
Published online January 1998 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/5.6.355

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In this paper, we report the sequences of 100 cDNA clones newly determined from a set of sizefractionated human brain cDNA libraries and predict the coding sequences of the corresponding genes, named KIAA0819 to KIAA0918. These cDNA clones were selected on the basis of their coding potentials of large proteins (50 kDa and more) by using in vitro transcription/translation assays. The sequence data showed that the average sizes of the inserts and corresponding open reading frames are 4.4 kb and 2.5 kb (831 amino acid residues), respectively. Homology and motif/domain searches against the public databases indicated that the predicted coding sequences of 83 genes were similar to those of known genes, 59% of which (49 genes) were categorized as coding for proteins functionally related to cell signaling/communication, cell structure/motility and nucleic acid management. The chromosomal locations and the expression profiles of all the genes were also examined. For 54 clones including brain-specific ones, the mRNA levels were further examined among 8 brain regions (amygdala, corpus callosum, cerebellum, caudate nucleus, hippocampus, substantia nigra, subthalamic nucleus, and thalamus), spinal cord, and fetal brain.

Keywords: large proteins; in vitro transcription/translation; cDNA sequencing; expression profile; chromosomal location; brain

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Subjects: Genetics and Genomics

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