Journal Article

Characterization of a <i>Caenorhabditis elegans recA</i>-like Gene <i>Ce-rdh-1</i> Involved in Meiotic Recombination

Takako Takanami, Shusei Sato, Takeshi Ishihara, Isao Katsura, Hideyuki Takahashi and Atsushi Higashitani

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 5, issue 6, pages 373-377
Published in print January 1998 | ISSN: 1340-2838
Published online January 1998 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/5.6.373

Show Summary Details

Preview

A recA-like gene was identified in the Caenorhabditis elegans genome project database. The putative product of the gene, termed Ce-rdh-1 (C. elegans RAD51 and DMC1/LIM15 homolog 1), consists of 357 amino acid residues. The predicted amino acid sequence of Ce-rdh-1 showed 46–60% identity to both RAD51 type and DMC1/LIM15 type genes in severaleuk aryote species. The results of RNAi (RNA-mediated interference) indicated that repression of Ce-rdh-1 blocked chromosome condensation of six bivalents and dissociation of chiasmata in oocytes of F1 progeny. Oogenesis did not proceed to the diakinesis stage. Accordingly, all the eggs produced (F2) died in early stages. These results suggest that Ce-rdh-1 participates in meiotic recombination.

Keywords: recA-like gene; DMC1; LIM15; RAD51; meiosis

Journal Article.  0 words. 

Subjects: Genetics and Genomics

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.