Journal Article

Nucleus-encoded Precursors to Thylakoid Lumen Proteins of <i>Euglena gracilis</i> Possess Tripartite Presequences

Rostislav Vacula, Jürgen M. Steiner, Juraj Krajcovic, Libor Ebringer and Wolfgang Löffelhardt

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 6, issue 1, pages 45-49
Published in print January 1999 | ISSN: 1340-2838
Published online January 1999 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/6.1.45

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The complete presequences of the nucleus-encoded precursors to two proteins, cytochrome c6 and the 30-kDa protein of the oxygen-evolving complex, that reside in the thylakoid lumen of the chloroplasts of Euglena gracilis are presented. Sorting of these proteins involves translocation across four membranes, the three-membraned chloroplast envelope and the thylakoid membrane. The tripartite presequences show the structure: signal sequence — transit sequence — signal sequence. Three hydrophobic domains become apparent: two of them correspond to signal sequences for translocation across the endoplasmic reticulum (ER) membrane and the thylakoid membrane, respectively, whereas the third constitutes the stop-transfer signal contained in the long stroma-targeting part of the tripartite presequence.

Keywords: Euglena gracilis; petJ; protein import; psbO; thylakoid lumen; tripartite presequences

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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