Journal Article

Characterization of a Single-Copy <i>Arabidopsis</i> Gene Encoding a Protein Showing Limited Similarity to the <i>N</i>-terminus of the Mammalian Clathrin-Assembly Protein AP180

Rajeev Gupta and John C. Gray

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 6, issue 1, pages 51-55
Published in print January 1999 | ISSN: 1340-2838
Published online January 1999 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/6.1.51

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The Arabidopsis 194 gene encoding a protein containing sequence similarity to an N-terminal region of the clathrin-assembly protein AP180 has been identified in a 4.9-kb region of genomic DNA upstream of the gene encoding the high mobility group protein HMG-I/Y. The gene consists of 12 exons and 11 introns, identified by comparison with partial cDNAs and using the NetPlantGene programme, and encodes a protein of 584 amino acid residues. The C-terminal region of the protein contains 8 tandem repeats of a 17-amino-acid-residue sequence. Southern blot analysis of genomic DNA from Columbia and Landsberg ecotypes of Arabidopsis indicates the presence of a single copy of the 194 gene. The 194 gene is expressed in all organs of Arabidopsis including roots, stems, leaves, flowers, and developing siliques, as determined by northern blot analysis.

Keywords: Arabidopsis thaliana; clathrin; clathrin assembly protein AP180; high mobility group protein HMG-I/Y; Mycoplasma pulmonis variable surface antigen

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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