Journal Article

The Gene for an Exopolyphosphatase of <i>Pseudomonas aeruginosa</i>

Tatsushi Miyake, Toshikazu Shiba, Atsushi Kameda, Yoshiharu Ihara, Masanobu Munekata, Kazuya Ishige and Toshitada Noguchi

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 6, issue 2, pages 103-108
Published in print January 1999 | ISSN: 1340-2838
Published online January 1999 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/6.2.103

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In Pseudomonas aeriginosa, a gene, ppx, that encodes exopolyphosphatase [exopoly(P)ase; EC 3.6.1.11] of 506 amino acids (56,419 Da) was found downstream of the gene for polyphosphate kinase, ppk. Since ppx is located in the opposite direction of the ppk gene, they do not constitute an operon. The predicted amino acid sequence of PPX is 41% identical with Escherichia coli PPX. The gene product of ppx(paPPX) was overproduced in E. coli, and its activity was evaluated. Orthophosphate (Pi) is released from polyphosphate [poly(P)], the average chain lengths of which are 79 and 750, respectively. The amount of Pi released matched the amount of poly(P) lost. Thus ppx encodes an enzyme that has exopoly(P)ase activity.

Keywords: Exopolyphosphatase; inorganic polyphosphate; Pseudomonas aeruginosaPAO1

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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