Journal Article

Cloning of Cellulose Synthase Genes from <i>Acetobacter xylinum</i> JCM 7664: Implication of a Novel Set of Cellulose Synthase Genes

Yoko Umeda, Atsushi Hirano, Michio Ishibashi, Hideo Akiyama, Takuo Onizuka, Masahiko Ikeuchi and Yorinao Inoue

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 6, issue 2, pages 109-115
Published in print January 1999 | ISSN: 1340-2838
Published online January 1999 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/6.2.109

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Three sets of cellulose synthase genes were cloned froma cellulose-producing bacterium Acetobacter xylinum JCM 7664. One set of genes (bcsAI/bcsBI/bcsCI/bcsDI) were highly conserved with the wellestablished type I genes in other strains of A. xylinum, while the other two (bcsABII-A, bcsABII-B) were homologous to the known type II (acsAII). Unexpectedly, they were immediately followed by a gene cluster of bcsX/bcsY/bcsCII/ORF569, likely forming an operon. Western blotting demonstrated that the BcsY protein accumulated in cells. Since BcsY showed striking similarities to a number of membrane-bound transacylases, it was hypothesized that the type II cellulose synthase produces acylated cellulose, which might be anchored on the cytoplasmic membrane. An insertion sequence of IS1380-type was found just upstream of the one type II gene (bcsABII-B), suggestive of nonfunctioning.

Keywords: cellulose synthase; transacylase; Acetobacter xylinum; transposon; IS1380

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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