Journal Article

Sequence Analysis of a Total of Three Megabases of DNA in Two Regions of Chromosome 8p

Minoru Isomura, Shiro Ikegawa, Takao Kinjo, Kumiko Takeuchi, Yuka Yamane-Tanaka, Kikuko Kitami and Yusuke Nakamura

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 6, issue 6, pages 387-399
Published in print January 1999 | ISSN: 1340-2838
Published online January 1999 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/6.6.387

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Large-scale sequencing of genomic regions and in silico gene trapping together represent a highly efficient and powerful approach for identifying novel genes. We performed megabase-level sequence analyses of two genomic regions on human chromosome 8p (8p11.2 and 8p22.→p21.3), after covering those segments with sequence-ready contigs composed of 74 cosmids, 14 BACs, and three PAC clones. We determined continuous nucleotide sequences of 1,856,753 bases on 8p11.2 and 1,210,381 bases on 8p22→p21.3 by combining the shotgun and primer-walking methods. In silico gene trapping identified four novel genes in the 8p11.2 region and, in the 8p22→p21.3 region, six known genes (PRLTS, PCM1, MTMR7, HCAT2, HFREP-1 and PHP) and three novel genes. The distribution of Alu and LINE1 repetitive elements and the densities of predicted exons were different in each region, and Alu-rich portions contained more exonic sequences than LINE1-rich areas.

Keywords: large-scale DNA sequencing; physical and transcriptional maps; human chromosome 8p11.2; human chromosome 8p22→p21.3

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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