Journal Article

Sequence-ready 1-Mb YAC, BAC and Cosmid Contigs Covering the Distal Imprinted Region of Mouse Chromosome 7

Reiko Kato, Hisao Shirohzu, Takaaki Yokomine, Shin-ichi Mizuno, Tsunehiro Mukai and Hiroyuki Sasaki

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 6, issue 6, pages 401-405
Published in print January 1999 | ISSN: 1340-2838
Published online January 1999 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/6.6.401

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We have constructed approximately 1-Mb contigs of yeast artificial chromosome (YAC), bacterial artificial chromosome (BAC) and cosmid clones covering the imprinted region in mouse chromosome band 7F4/F5. This region is syntenic to human chromosome 11p15.5, which is associated with Beckwith-Wiedemann syndrome (BWS) and certain childhood and adult tumors. These contigs provide the basis for genomic sequencing, identification of genes and their regulatory elements, and functional studies in transgenic and knockout mice, which should be of help to understand not only the mechanisms of imprinting but also the molecular events involved in the genesis of BWS and tumors.

Keywords: genomic imprinting; mouse chromosome 7; YAC contig; BAC contig; cosmid contig; Beckwith-Wiedemann syndrome

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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