Journal Article

Tagged Mutagenesis and Gene-trap in the Moss, <i>Physcomitrella patens</i> by Shuttle Mutagenesis

Tomoaki Nishiyama, Yuji Hiwatashi, Keiko Sakakibara, Masahiro Kato and Mitsuyasu Hasebe

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 7, issue 1, pages 9-17
Published in print January 2000 | ISSN: 1340-2838
Published online January 2000 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/7.1.9

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The moss, Physcomitrella patens has been used as a useful material in many fields, because of its simple body plan, ease of gene targeting, and other reasons. Although many mutants have been reported, no method to isolate the corresponding genes was reported. We developed a gene tagging and gene-trap system in P. patens by using the shuttle mutagenesis technique, which has been used in the budding yeast. In 5264 tagged lines, 203 mutants with altered developmental or morphological phenotypes were obtained. In 129 of 4757 gene-trap lines, β-glucuronidase (GUS) activity was detected in some tissue. Although multiple copies of a tag were detected in many tagged lines by Southern analyses, most copies are likely integrated at the same locus according to PCR analyses.

Keywords: tagging; gene-trap; Physcomitrella patens; homologous recombination; shuttle mutagenesis

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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