Journal Article

<i>C. elegans</i> KLP-11/OSM-3/KAP-1: Orthologs of the Sea Urchin Kinesin-II, and Mouse KIF3A/KIFB/KAP3 Kinesin Complexes

M. Liakot Ali Khan, M. Yusuf Ali, Zeba K. Siddiqui, M. Afaq Shakir, Hirohito Ohnishi, Ken Nishikawa and Shahid S. Siddiqui

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 7, issue 2, pages 121-125
Published in print January 2000 | ISSN: 1340-2838
Published online January 2000 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/7.2.121

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Kinesins are intracellular multimeric transport motor proteins that move cellular cargo on microtubule tracks. It has been shown that the sea urchin KRP85/95 holoenzyme associates with a KAP115 non-motor protein, forming a heterotrimeric complex in vitro, called the Kinesin-II. Here we describe isolation of a cDNA clone corresponding to the klp-11 kinesin in C. elegans. Our sequence analysis of the encoded KLP-11 shows that it shares high homology with the OSM-3 kinesin. We also describe a nematode cDNA encoding KAP-1 that shares extensive homology with the sea urchin KAP115 kinesin associated protein. Sequencebased structural analysis of the OSM-3, KLP-11, and KAP-1, presented here suggests that these may form a heterotrimeric complex. We also describe the presence of a Drosophila armadillo consensus motif in CeKAP-1, first found in spKAP115, that suggests a possible role for the KAP-1 in signal transduction.

Keywords: C. elegans; OSM-3/KLP-11/KAP-1; Kinesin-II; armadillo motif

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Subjects: Genetics and Genomics

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