Journal Article

Analysis of Unique Variable Region of a Plant Root Inducing Plasmid, pRi1724, by the Construction of its Physical Map and Library

Kazuki Moriguchi, Yasunobu Maeda, Megumi Satou, Miho Kataoka, Nobukazu Tanaka and Kazuo Yoshida

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 7, issue 3, pages 157-163
Published in print January 2000 | ISSN: 1340-2838
Published online January 2000 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/7.3.157

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Ri plasmids in Agrobacterium rhizogenes specifically induce the hairy root syndrome on various dicotyledonous plants. Its T-DNA transfer system as well as those of Ti plasmids have successfully provided the fundamental technique to introduce exogenous genes into plants. To study the Ri genome structure, we constructed a complete BamHI physical map and a lambda library of pRi1724 of A. rhizogenes strain 1724. By using these, we carried out the complete sequence of the 74-kb region between the right border of TDNA and tra operon, which is the highly variable region (VAR) among Ri and Ti plasmids. As a result, we found three kinds of putative ABC-type transport operons, histidine utilization operon, glycerol utilization operon and two chemoreceptor genes. In addition, a virulence-related gene, tzs was located independently of the vir region.

Keywords: Ri plasmid; Agrobacterium rhizogenes; Physical map; VAR (variable region)

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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