Journal Article

Comparison of Expressed Sequence Tags from Male and Female Sexual Organs of <i>Marchantia polymorpha</i>

Rie Nishiyama, Katsuyuki T. Yamato, Kenji Miura, Megumi Sakaida, Sachiko Okada, Kaoru Kono, Masayoshi Takahama, Takefumi Sone, Mizuki Takenaka, Hideya Fukuzawa and Kanji Ohyama

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 7, issue 3, pages 165-174
Published in print January 2000 | ISSN: 1340-2838
Published online January 2000 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/7.3.165

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A total of 935 expressed sequence tags (ESTs) from male immature sexual organ were determined, of which 600 ESTs were assembled into 110 non-redundant groups, resulting in 445 unique EST sequences. Of these, 244 sequences shared significant similarities to known nucleotide or amino acid sequences in other organisms. The remaining 201 unique sequences showed no significant matches and thus are likely to be novel transcripts. ESTs from male and female immature sexual organs of a liverwort, Marchantia polymorpha, were compared to characterize gene expression patterns during sex differentiation. Ninety-nine male ESTs turned out to be common genes found also in the female library. Interestingly, one of the ESTs found only in male shows a significant similarity to the transformer-2 gene involved in sex determination in Drosophila. In female, several unique lectin ESTs were found that are not present in the male library.

Keywords: Marchantia polymorpha; EST; male sexual organ; sex differentiation; transformer-2

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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