Journal Article

Analysis of Expressed Sequence Tags of Flower Buds in <i>Lotus japonicus</i>

Makoto Endo, Takao Kokubun, Yoshihito Takahata, Atsushi Higashitani, Satoshi Tabata and Masao Watanabe

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 7, issue 3, pages 213-216
Published in print January 2000 | ISSN: 1340-2838
Published online January 2000 | e-ISSN: 1756-1663 | DOI:

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In order to study gene expression in a reproductive organ, we constructed a cDNA library of mature flower buds in Lotus japonicus, and characterized expressed sequence tags (ESTs) of 842 clones randomlyselected. The EST sequences were clustered into 718 non-redundant groups. From BLAST and FASTA search analyses of both protein and DNA databases, 58.5% of the EST groups showed significant sequence similarities to known genes. Several genes encoding these EST clones were identified as pollen-specific genes, such as pectin methylesterase, ascorbate oxidase, and polygalacturonase, and as homologous genes involved in pollen-pistil interaction. Comparison of these EST sequences with those derived from the whole plant of L. japonicus, revealed that 64.8% of EST sequences from the flower buds were not found in EST sequences of the whole plant. Taken together, the EST data from flower buds generated in this study is useful in dissecting gene expression in floral organ of L. japonicus.

Keywords: Legume; Lotus japonicus; ESTs; flower bud; pollen-specific gene

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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