Journal Article

Structural Analysis of <i>Arabidopsis thaliana</i> Chromosome 3. II. Sequence Features of the 4,251,695 bp Regions Covered by 90 P1, TAC and BAC Clones

Takakazu Kaneko, Tomohiko Katoh, Shusei Sato, Yasukazu Nakamura, Erika Asamizu and Satoshi Tabata

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 7, issue 3, pages 217-221
Published in print January 2000 | ISSN: 1340-2838
Published online January 2000 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/7.3.217

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To deduce the entire sequence of the top arm of the Arabidopsis thaliana chromosome 3, the sequence determination was performed on a total of 90 P1, TAC and BAC clones chosen according to our sequencing strategy. Sequence features of the resulting 4,251,695 bp regions were analyzed with various computer programs for similarity search and gene modeling. As a result, a total of 941 potential protein-coding genes were identified. The average density of the genes identified was 1 gene per 4210 bp. Introns were observed in 73% of the genes, and the average number per gene and the average length of the introns were 3.6 and 159 bp, respectively. These sequence features are essentially identical to those of chromosomes 3and 5 in our previous reports. The regions also contained 14 tRNA genes when searched by similarity to reported tRNA genes and the tRNA scan-SE program. The sequence data and information on the potential genes are available through the World Wide Web database KAOS (Kazusa Arabidopsis data Opening Site) at .

Keywords: Arabidopsis thaliana chromosome 3; genomic sequence; P1 genomic library; TAC genomic library; gene prediction

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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