Journal Article

Generation of 10,154 Expressed Sequence Tags from a Leafy Gametophyte of a Marine Red Alga, <i>Porphyra yezoensis</i>

Itoshi Nikaido, Erika Asamizu, Maiko Nakajima, Yasukazu Nakamura, Naotsune Saga and Satoshi Tabata

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 7, issue 3, pages 223-227
Published in print January 2000 | ISSN: 1340-2838
Published online January 2000 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/7.3.223

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A total of 10,154 5′-end expressed sequence tags (EST) were established from the normalized and size-selected cDNA libraries of a marine red alga, Porphyra yezoensis. Among the ESTs, 2140 were unique species, and the remaining 8014 were grouped into 1127 species. Database search of the 3267 non-redundant ESTs by BLAST algorithm showed that the sequences of 1080 species (33.1%) have similarity to those of registered genes from various organisms including higher plants, mammals, yeasts, and cyanobacteria,while 2187 (66.9%) are novel. Codon usage analysis in the coding regions of 101 non-redundant EST groups showing significant similarity to known genes indicated the higher GC contents at the third position of codons (79.4%) than the first (62.2%) and the second position (45.0%), suggesting that the genome hasbeen exposed to high GC pressure during evolution. The sequence data of individual ESTs are available at the web site .

Keywords: Porphyra yezoensis; Rhodophyta; red alga; EST; codon usage

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Subjects: Genetics and Genomics

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