Journal Article

Cloning and Sequencing of the Levansucrase Gene from <i>Acetobacter xylinum</i> NCI 1005

Kenji Tajima, Toshiyuki Tanio, Yasunobu Kobayashi, Hiroyuki Kohno, Masashi Fujiwara, Toshikazu Shiba, Tomoki Erata, Masanobu Munekata and Mitsuo Takai

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 7, issue 4, pages 237-242
Published in print January 2000 | ISSN: 1340-2838
Published online January 2000 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/7.4.237

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The levansucrase gene (lsxA) was cloned from the genomic DNA of Acetobacter xylinum NCI 1005, and the nucleotide sequence of the lsxA gene (1,293 bp) was determined. The deduced amino acid sequenceof the lsxA gene showed 57.4% and 46.2% identity with the levansucrases from Zymomonas mobilis and Erwinia amylovora, respectively, while only 35.2% identity with that from Acetobacter diazotrophicus. Thegene product of lsxA (LsxA) that was overproduced in E. coli coded for a polypeptide of molecular mass 47 kDa. The LsxA released glucose and produced polysaccharide from sucrose, the structure of which was analyzed by nuclear magnetic resonance spectroscopy and determined to be a β-(2,6)-linked polyfructan. From these results, it can be concluded that the lsxA gene encodes A. xylinum levansucrase.

Keywords: Levansucrase gene; DNA sequencing; Acetobacter xylinum

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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