Journal Article

Genes Encoding Nitrilase-like Proteins from Tobacco

Mitsuko Dohmoto, Hiroyuki Tsunoda, Gen Isaji, Ryo Chiba and Kazuo Yamaguchi

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 7, issue 5, pages 283-289
Published in print January 2000 | ISSN: 1340-2838
Published online January 2000 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/7.5.283

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Nitrilase (nitrile aminohydrolase, EC 3.5.5.1) catalyzes the hydrolysis of indole-3-acetonitrile (IAN) to indole-3-acetic acid (IAA). Arabidopsis thaliana genome has four nitrilase genes (NIT1, NIT2, NIT3 and NIT4). Three (NIT1, NIT2 and NIT3) of the four genes have high similarity. We have cloned two NIT4 homologs (TNIT4A and TNIT4B) from tobacco (Nicotiana tabacum). Genomic Southern hybridization, among other experiments, strongly suggests that tobacco has NIT4 homologs but not NIT1 to NIT3 homologs. Introduction of Arabidopsis NIT2 into tobacco conferred IAN-mediated growth inhibition, probably due to hydrolysis of IAN to IAA, while ectopic expression of TNIT4A had little effect on the sensitivity of transgenic plants to IAN. Nitrilase activity of TNIT4 proteins is discussed.

Keywords: Nicotiana tabacum; nitrilase; indole-3-acetic acid; indole-3-acetonitrile; transgenic plant

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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