Journal Article

Generation of Expressed Sequence Tags from Low-CO<sub>2</sub> and High-CO<sub>2</sub> Adapted Cells of <i>Chlamydomonas reinhardtii</i>

Erika Asamizu, Kenji Miura, Kenichi Kucho, Yoshihiro Inoue, Hideya Fukuzawa, Kanji Ohyama, Yasukazu Nakamura and Satoshi Tabata

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 7, issue 5, pages 305-307
Published in print January 2000 | ISSN: 1340-2838
Published online January 2000 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/7.5.305

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To characterize genes whose expression is induced in carbon-stress conditions, 12,969 and 13,450 5′-end expressed sequence tags (ESTs) were generated from cells grown in low-CO2 and high-CO2 conditions ofthe unicellular green alga, Chlamydomonas reinhardtii. These ESTs were clustered into 4436 and 3566 non-redundant EST groups, respectively. Comparison of their sequences with those of 3433 non-redundant ESTs previously generated from the cells under the standard growth condition indicated that 2665 and 1879 EST groups occurred only in the low-CO2 and high-CO2 populations, respectively. It was also noted that 96.2% and 96.0% of the cDNA species respectively obtained from the low-CO2 and high-CO2 conditions had no similar EST sequence deposited in the public databases. The EST species identified only in the low-CO2 treated cells included genes previously reported to be expressed specifically in low-CO2 acclimatized cells, suggesting that the ESTs generated in this study will be a useful source for analysis of genes related to carbon-stress acclimatization. The sequence information and search results of each clone will appear at the web site: .

Keywords: Chlamydomonas reinhardtii; cDNA; EST; CO2 stress

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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