Journal Article

Characterization of Four Extensin Genes in <i>Arabidopsis thaliana</i> by Differential Gene Expression under Stress and Non-Stress Conditions

Yoshu Yoshiba, Chisako Aoki, Satoshi Iuchi, Tokihiko Nanjo, Motoaki Seki, Fumihiko Sekiguchi, Kazuko Yamaguchi-Shinozaki and Kazuo Shinozaki

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 8, issue 3, pages 115-122
Published in print January 2001 | ISSN: 1340-2838
Published online January 2001 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/8.3.115

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From Arabidopsis thaliana we isolated four different cDNAs that encode extensins, a family of cell-wall hydroxyproline-rich glycoproteins (HRGPs). Putative proteins (AtExt2–5) contained one open reading frame and characteristic Ser-(Pro)4 sequences organized in a high-order repetitive motif. AtExt2–5 genes were strongly expressed during rehydration after dehydration. They were also expressed after treatment with various amino acids. In particular, AtExt3 and five mRNAs were abundantly accumulated after treatment with l-Ser, Hyp, and l-Pro, which are major components ofextensin proteins. The AtExt transcripts were strongly expressed in root tissues of both unbolted and bolted plants. The transcripts of AtExt2, 3, and 5 were also detected in the lower stem and flower buds, and that of AtExt4 was detected in bolted flowers. Therefore, we suggest that these four AtExt genes are novel extensin genes in A. thaliana, because the expression of atExt1, which has already been isolated from A. thaliana, was different from these.

Keywords: Arabidopsis thaliana; extensin; hydroxyproline-rich glycoprotein (HRGP); water stress; root

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Subjects: Genetics and Genomics

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