Journal Article

Complete Genome Sequence of an Aerobic Thermoacidophilic Crenarchaeon, <i>Sulfolobus tokodaii</i> strain7

Yutaka Kawarabayasi, Yumi Hino, Hiroshi Horikawa, Koji Jin-no, Mikio Takahashi, Mitsuo Sekine, Sin-ichi Baba, Akiho Ankai, Hiroki Kosugi, Akira Hosoyama, Shigehiro Fukui, Yoshimi Nagai, Keiko Nishijima, Rie Otsuka, Hidekazu Nakazawa, Minako Takamiya, Yumiko Kato, Takio Yoshizawa, Toshihiro Tanaka, Yutaka Kudoh, Jun Yamazaki, Norihiro Kushida, Akio Oguchi, Ken-ichi Aoki, Sayaka Masuda, Masao Yanagii, Masami Nishimura, Akihiko Yamagishi, Tairo Oshima and Hisasi Kikuchi

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 8, issue 4, pages 123-140
Published in print January 2001 | ISSN: 1340-2838
Published online January 2001 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/8.4.123

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The complete genomic sequence of an aerobic thermoacidophilic crenarchaeon, Sulfolobus tokodaii strain7 which optimally grows at 80°C, at low pH, and under aerobic conditions, has been determined by the whole genome shotgun method with slight modifications. The genomic size was 2,694,756 bp long and the G+C content was 32.8%. The following RNA-coding genes were identified: a single 16S–23S rRNA cluster, one 5S rRNA gene and 46 tRNA genes (including 24 intron-containing tRNA genes). The repetitive sequences identified were SR-type repetitive sequences, long dispersed-type repetitive sequences and Tn-like repetitive elements. The genome contained 2826 potential protein-coding regions (open reading frames, ORFs). By similarity search against public databases, 911 (32.2%) ORFs were related to functional assigned genes, 921 (32.6%) were related to conserved ORFs of unknown function, 145 (5.1%) contained some motifs, and remaining 849 (30.0%) did not show any signi.cant similarity to the registered sequences. The ORFs with functional assignments included the candidate genes involved in sulfide metabolism, the TCA cycle and the respiratory chain. Sequence comparison provided evidence suggesting the integration of plasmid, rearrangement of genomic structure, and duplication of genomic regions that may be responsible for the larger genomic size of the S. tokodaii strain7 genome. The genome contained eukaryote-type genes which were not identified in other archaea and lacked the CCA sequence in the tRNA genes. The result suggests that this strain is closer to eukaryotes among the archaea strains so far sequenced.

The data presented in this paper are also available on the internet homepage ().

Keywords: aerobic thermoacidophilic crenarchaeon; genome sequencing; whole genome shotgun method; comparative analysis; plasmid

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Subjects: Genetics and Genomics

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