Journal Article

Monitoring of Methyl Jasmonate-responsive Genes in Arabidopsis by cDNA Macroarray: Self-activation of Jasmonic Acid Biosynthesis and Crosstalk with Other Phytohormone Signaling Pathways

Yuko Sasaki, Erika Asamizu, Daisuke Shibata, Yasukazu Nakamura, Takakazu Kaneko, Koichiro Awai, Masayuki Amagai, Chikara Kuwata, Taneaki Tsugane, Tatsuru Masuda, Hiroshi Shimada, Ken-ichiro Takamiya, Hiroyuki Ohta and Satoshi Tabata

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 8, issue 4, pages 153-161
Published in print January 2001 | ISSN: 1340-2838
Published online January 2001 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/8.4.153

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Jasmonates mediate various physiological events in plant cells such as defense responses, flowering, and senescence through intracellular and intercellular signaling pathways, and the expression of a large number of genes appears to be regulated by jasmonates. In order to obtain information on the regulatory network of jasmonate-responsive genes (JRGs) in Arabidopsis thaliana (Arabidopsis), we screened 2880 cDNA clones for jasmonate responsiveness by a cDNA macroarray procedure. Since many of the JRGs reported so far have been identified in leaf tissues, the cDNA clones used were chosen from a non-redundant EST library that was prepared from above-ground organs. Hybridization to the filters was achieved using α-33P-labeled single-strand DNAs synthesized from mRNAs obtained from methyl jasmonate (MeJA)-treated and untreated Arabidopsis seedlings. Data analysis identified 41 JRGs whose mRNA levels were changed by more than three fold in response to MeJA. This was confirmed by Northern blot analysis by using eight representatives. Among the 41 JRGs identified, 5 genes were JA biosynthesis genes and 3 genes were involved in other signaling pathways (ethylene, auxin, and salicylic acid). These results suggest the existence of a positive feedback regulatory system for JA biosynthesis and the possibility of crosstalk between JA signaling and other signaling pathways.

Keywords: Arabidopsis; cDNA macroarray; DNA array; jasmonic acid; methyl jasmonate

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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