Journal Article

Comparison of Sequences of cDNA Clones Obtained from Oligo-capping cDNA Libraries with Those from UniGene

Tetsuo Nishikawa, Toshio Ota, Yuri Kawai, Shizuko Ishii, Kaoru Saito, Jun-ichi Yamamoto, Ai Wakamatsu, Masashi Ozawa, Yutaka Suzuki, Sumio Sugano and Takao Isogai

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 8, issue 6, pages 255-262
Published in print January 2001 | ISSN: 1340-2838
Published online January 2001 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/8.6.255

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We compared in detail the characteristics of the sequences of the cDNA clones obtainedb y the oligo-capping method(oligo-capping clones) with that of the sequences in the UniGene database. To compare the completeness of the sequences, three new variables, “fullness-proportion of clones” (the ratio of complete clones to total clones in a library), “fullness-proportion of genes” (the ratio of complete genes to total genes in a library), and “fullness-prop ortion of database” (the ratio of complete genes to total genes in a database sampled from a library), were defined. The fullness-proportion of clones of oligo-capping clones was 57.3%, 2.2 times larger than that of UniGene (25.9%). The fullness-proportion of genes of oligo-capping clones was 41.8%, 2.4 times larger than that of UniGene (17.8%). When gene length was restricted to ≥ 1.5 kb, the fullness-proportion of genes of oligo-capping clones was four times larger than that of UniGene. The fullness-proportion of database of oligo-capping clones was approximately the same as that of UniGene. By simulating the clone redundancy, this coincidence was found to be due to the large redundancy of the UniGene database. Consequently, the cDNA sequence database of oligo-capping clones enabledhigh throughput selection of full-length cDNA clones.

Keywords: database; full-length cDNA; UniGene; ESTs; oligo-capping

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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