Journal Article

Characterization of Genes Encoding Multi-domain Proteins in the Genome of the Filamentous Nitrogen-fixing Cyanobacterium <i>Anabaena</i> sp. Strain PCC 7120

Masayuki Ohmori, Masahiko Ikeuchi, Naoki Sato, Peter Wolk, Takakazu Kaneko, Teruo Ogawa, Minoru Kanehisa, Susumu Goto, Shuichi Kawashima, Shinobu Okamoto, Hidehisa Yoshimura, Hiroshi Katoh, Takatomo Fujisawa, Shigeki Ehira, Ayako Kamei, Shizue Yoshihara, Rei Narikawa and Satoshi Tabata

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 8, issue 6, pages 271-284
Published in print January 2001 | ISSN: 1340-2838
Published online January 2001 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/8.6.271

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Computational analysis of gene structures in the genome of Anabaena sp. PCC 7120 revealed the presence of a large number of genes encoding proteins with multiple functional domains. This was most evident in the genes for signal transduction pathway and the related systems. Comparison of the putative amino acid sequences of the gene products with those in the Pfam database indicated that GAF and PAS domains which may be involved in signal recognition were extremely abundant in Anabaena: 87 GAF domains in 62 ORFs and 140 PAS domains in 59 ORFs. As for the two-component signal transduction system, 73, 53, and 77 genes for simple sensory His kinases, hybrid His kinases and simple response regulators, respectively, many of which contained additional domains of diverse functions, were presumptively assigned. A total of 52 ORFs encoding putative Hanks-type Ser/Thr protein kinases with various domains such as WD-repeat, GAF and His kinase domains, as well as genes for presumptive protein phosphatases, were also identified. In addition, genes for putative transcription factors and for proteins in the cAMP signal transduction system harbored complex gene structures with multiple domains.

Keywords: GAF domain; multi-domain protein; PAS domain; photoreceptor domain; protein kinase; two-component system

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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