Journal Article

Construction of a Genetic Linkage Map of the Model Legume <i>Lotus japonicus</i> Using an Intraspecific F<sub>2</sub> Population

Masaki Hayashi, Akira Miyahara, Shusei Sato, Tomohiko Kato, Makoto Yoshikawa, Michiko Taketa, Makoto Hayashi, Andrea Pedrosa, Ryutaku Onda, Haruko Imaizumi-Anraku, Andreas Bachmair, Niels Sanda, Jens Stougaard, Yoshikatsu Murooka, Satoshi Tabata, Shinji Kawasaki, Masayoshi Kawaguchi and Kyuya Harada

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 8, issue 6, pages 301-310
Published in print January 2001 | ISSN: 1340-2838
Published online January 2001 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/8.6.301

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Among leguminous plants, the model legume Lotus japonicus (Regel) Larsen has many biological and genetic advantages. We have developed a genetic linkage map of L. japonicus based on amplified fragment length polymorphism (AFLP), simple sequence repeat polymorphism (SSRP) and derived cleaved amplified polymorphic sequence (dCAPS). The F2 mapping population used was derived from a cross between two L. japonicus accessions Gifu B-129 and Miyakojima MG-20. These parental accessions showed remarkable cytological differences, particularly with respect to size and morphology of chromosomes 1 and 2. Using fluorescence in situ hybridization (FISH) with BAC clones from Gifu B-129 and TAC (Transformation-competent Artificial Chromosome) clones from Miyakojima MG-20, a reciprocal translocation was found to be responsible for the cytological differences between chromosomes 1 and 2. The borders of the translocations were identified by FISH and by alignment toward the L. filicaulis × L. japonicus Gifu B-129 linkage map. The markers from the main translocated region were located on linkage groups 1 and 2 of the two accessions, Gifu B-129 and Miyakojima MG-20, respectively. The framework of the linkage map was constructed based on codominant markers, and then dominant markers were integrated separately in each linkage group of the parents. The resulting linkage groups correspond to the six pairs of chromosomes of L. japonicus and consist of 287 markers with 487.3 cM length in Gifu B-129 and 277 markers with 481.6 cM length in Miyakojima MG-20. The map and marker information is available through the World Wide Web at .

Keywords: Lotus japonicus; linkage map; translocation; AFLP; SSR; dCAPS; FISH

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Subjects: Genetics and Genomics

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