Journal Article

Binding of 5-bromouracil-containing S/MAR DNA to the Nuclear Matrix

Hideki Ogino, Michihiko Fujii, Wataru Satou, Toshikazu Suzuki, Eriko Michishita and Dai Ayusawa

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 9, issue 1, pages 25-29
Published in print January 2002 | ISSN: 1340-2838
Published online January 2002 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/9.1.25

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Substitution of thymine with 5-bromouracil in DNA is known to change interaction between DNA and proteins, thereby inducing various biological phenomena. We hypothesize that A/T-rich scaffold/nuclear matrix attachment region (S/MAR) sequences are involved in the effects of 5-bromodeoxyuridine. We examined an interaction between DNA containing an intronic S/MAR sequence of the immunoglobulin heavy chain gene and nuclear halos prepared from HeLa cells. Upon substitution with 5-bromouracil, the S/MAR DNA bound more tightly to the nuclear halos. The multi-functional nuclear matrix protein YY1 was also found to bind more strongly to 5-bromouracil-substituted DNA containing its recognition motif. These results are consistent with the above hypothesis.

Keywords: BrdU; 5-bromouracil; S/MAR sequence; nuclear matrix; IgH gene; YY1

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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