Journal Article

Search for and Analysis of Single Nucleotide Polymorphisms (SNPs) in Rice (<i>Oryza sativa</i>, <i>Oryza rufipogon</i>) and Establishment of SNP Markers

Shinobu Nasu, Junko Suzuki, Rieko Ohta, Kana Hasegawa, Rika Yui, Noriyuki Kitazawa, Lisa Monna and Yuzo Minobe

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 9, issue 5, pages 163-171
Published in print January 2002 | ISSN: 1340-2838
Published online January 2002 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/9.5.163

Show Summary Details

Preview

We searched for SNPs in 417 regions distributed throughout the genome of three Oryza sativa ssp. japonica cultivars, two indica cultivars, and a wild rice (O. rufipogon). We found 2800 SNPs in approximately 250,000 aligned bases for an average of one SNP every 89 bp, or one SNP every 232 bp between two randomly selected strains. Graphic representation of the frequency of SNPs along each chromosome showed uneven distribution of polymorphism-rich and -poor regions, but little obvious association with the centromere or telomere. The 94 SNPs that we found between the closely related cultivars ‘Nipponbare’ and ‘Koshihikari’ can be converted into molecular markers. Our establishment of 213 co-dominant SNP markers distributed throughout the genome illustrates the immense potential of SNPs as molecular markers not only for genome research, but also for molecular breeding of rice.

Keywords: rice (Oryza sativa L., Oryza rufipogon); single nucleotide polymorphism (SNP); molecular marker; genome sequence; polymerase chain reaction (PCR)

Journal Article.  0 words. 

Subjects: Genetics and Genomics

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.