Journal Article

Stable Triple-Stranded DNA Formation and its Application to the SNP Detection

Yasushi Shigemori and Michio Oishi

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 12, issue 6, pages 441-449
Published in print February 2006 | ISSN: 1340-2838
Published online January 2005 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/dsi022

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We have found that a short stretch (30mer or larger) of triple-stranded DNA structure formed at the terminus (or very near) of linear DNA molecules is unusually stable, withstanding heat treatment at as high as 95°C. The stable triple-stranded structure is formed only when deoxyoligonucleotides are complementary to the strand terminating with 5′-phosphate and not to the strand terminating with 3′-OH. Presence of a single mismatched base in the complementary deoxyoligonucleotides drastically reduces the stability. We show that these unique properties of the terminal triple-stranded structure can be applied to the detection of single nucleotide polymorphisms in genomes without DNA dissociation and/or hybridization.

Keywords: RecA protein; stable triple-stranded DNA; deoxyoligonucleotide probe; SNPs detection

Journal Article.  4172 words.  Illustrated.

Subjects: Genetics and Genomics

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