Journal Article

Specific Enrichment of miRNAs in <i>Arabidopsis thaliana</i> Infected with <i>Tobacco mosaic virus</i>

Yuko Tagami, Naoko Inaba, Natsumaro Kutsuna, Yukio Kurihara and Yuichiro Watanabe

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 14, issue 5, pages 227-233
Published in print January 2007 | ISSN: 1340-2838
Published online December 2007 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/dsm022

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Abstract

RNA silencing is a broadly conserved machinery and is involved in many biological events. Small RNAs are key molecules in RNA silencing pathway that guide sequence-specific gene regulations and chromatin modifications. The silencing machinery works as an anti-viral defense in virus-infected plants. It is generally accepted that virus-specific small interfering (si) RNAs bind to the viral genome and trigger its cleavage. Previously, we have cloned and obtained sequences of small RNAs from Arabidopsis thaliana infected or uninfected with crucifer Tobacco mosaic virus. MicroRNAs (miRNAs) accumulated to a higher percentage of total small RNAs in the virus-infected plants. This was partly because the viral replication protein binds to the miRNA/miRNA* duplexes. In the present study, we mapped the sequences of small RNAs other than virus-derived siRNAs to the Arabidopsis genome and assigned each small RNA. It was demonstrated that only miRNAs increased as a result of viral infection. Furthermore, some newly identified miRNAs and miRNA candidates were found from the virus-infected plants despite a limited number of examined sequences. We propose that it is advantageous to use virus-infected plants as a source for cloning and identifying new miRNAs.

Keywords: Arabidopsis thaliana; Tobacco mosaic virus; microRNA; RNA silencing

Journal Article.  3411 words.  Illustrated.

Subjects: Genetics and Genomics

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