Journal Article

Transcriptome Analysis of a cDNA Library from Adult Human Epididymis

Jian-Yuan Li, Hai-Yan Wang, Jie Liu, Qiang Liu, Jin-Song Zhang, Feng-Chun Wan, Fu-Jun Liu, Shao-Hua Jin and Yong-Lian Zhang

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 15, issue 3, pages 115-122
Published in print June 2008 | ISSN: 1340-2838
Published online April 2008 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/dsn005

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Mammalian Gene Collection (MGC) verified over 9000 human full-ORF genes and FLJ Program reported 21 243 cDNAs of which 14 409 were unique ones and 5416 seemed to be protein-coding. The pity is that epididymis cDNA library was missing in their sequencing target list. Epididymis is a very important male accessory sex organ for sperm maturation and storage. Fully differentiated spermatozoa left from testis acquire their motility and capacity for fertilization via interactions with the epididymal epithelium duct lumen during passage through this convoluted duct. Here, we report that 20 000 clones from a healthy male epididymis cDNA library have been sequenced. The sequencing data provided 8234 known sequences and 650 unknown cDNA fragments. Hundred and six of 650 unknown cDNA clone inserts were randomly selected for fully sequencing. There were 25 unknown unique sequences and 19 released but unreported sequences came out. By northern blot analysis, four sequences randomly selected from the 19 released sequences with no known function showed positive mRNA signals in epididymis and testis. The signals for three of six from those unknown group showed as epididymis abundant in a region-specific manner but not in the testis and other tissues tested. All the sequencing data will be available on the website www.sdscli.com.

Keywords: human epididymis cDNA library; transcriptomes for human epididymis; sperm maturation

Journal Article.  3735 words.  Illustrated.

Subjects: Genetics and Genomics

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