Journal Article

Origins of Replication in <i>Sorangium cellulosum</i> and <i>Microcystis aeruginosa</i>

Feng Gao and Chun-Ting Zhang

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 15, issue 3, pages 169-171
Published in print June 2008 | ISSN: 1340-2838
Published online May 2008 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/dsn007

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The genome of Sorangium cellulosum has recently been completely sequenced, and it is the largest bacterial genome sequenced so far. In their report, Schneiker et al. (in Complete genome sequence of the myxobacterium Sorangium cellulosum, Nat. Biotechnol., 2007, 25, 1281–1289) concluded that ‘In the absence of the GC-skew inversion typically seen at the replication origin of bacterial chromosomes, it was not possible to discern the location of oriC’. In addition, the complete genome of Microcystis aeruginosa NIES-843 has also been recently sequenced, and in this report, Kaneko et al. (in Complete genomic structure of the bloom-forming toxic cyanobacterium Microcystis aeruginosa NIES-843, DNA Res., 2007, 14, 247–256) concluded that ‘there was no characteristic pattern, according to GC skew analysis’. Therefore, oriC locations of the above genomes remain unsolved. Using Ori-Finder, a recently developed computer program, in both genomes, we have identified candidate oriC regions that have almost all sequence hallmarks of bacterial oriCs, such as asymmetrical nucleotide distributions, being adjacent to the dnaN gene, and containing DnaA boxes and repeat elements.

Keywords: Sorangium cellulosum; Microcystis aeruginosa; origin of replication; Z-curve

Journal Article.  1020 words.  Illustrated.

Subjects: Genetics and Genomics

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