Journal Article

Genome-Wide Survey and Expression Analysis Suggest Diverse Roles of Glutaredoxin Gene Family Members During Development and Response to Various Stimuli in Rice

Rohini Garg, Shalu Jhanwar, Akhilesh K. Tyagi and Mukesh Jain

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 17, issue 6, pages 353-367
Published in print December 2010 | ISSN: 1340-2838
Published online November 2010 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/dsq023

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Glutaredoxins (GRXs) are glutathione-dependent oxidoreductase enzymes involved in a variety of cellular processes. In this study, our analysis revealed the presence of 48 genes encoding GRX proteins in the rice genome. GRX proteins could be classified into four classes, namely CC-, CGFS-, CPYC- and GRL-type, based on phylogenetic analysis. The classification was supported with organization of predicted conserved putative motifs in GRX proteins. We found that expansion of this gene family has occurred largely via whole genome duplication events in a species-specific manner. We explored rice oligonucleotide array data to gain insights into the function of GRX gene family members during various stages of development and in response to environmental stimuli. The comprehensive expression analysis suggested diverse roles of GRX genes during growth and development in rice. Some of the GRX genes were expressed in specific organs/developmental stages only. The expression of many of rice GRX genes was influenced by various phytohormones, abiotic and biotic stress conditions, suggesting an important role of GRX proteins in response to these stimuli. The identification of GRX genes showing differential expression in specific tissues or in response to environmental stimuli provide a new avenue for in-depth characterization of selected genes of importance.

Keywords: glutaredoxin; rice; development; stress; gene family

Journal Article.  7375 words.  Illustrated.

Subjects: Genetics and Genomics

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