Journal Article

Genopal™: A Novel Hollow Fibre Array for Focused Microarray Analysis

Daisuke Okuzaki, Tatsunobu Fukushima, Takahiro Tougan, Tomonori Ishii, Shigeto Kobayashi, Kazuyuki Yoshizaki, Takashi Akita and Hiroshi Nojima

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 17, issue 6, pages 369-379
Published in print December 2010 | ISSN: 1340-2838
Published online November 2010 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/dsq025

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Expression profiling of target genes in patient blood is a powerful tool for RNA diagnosis. Here, we describe Genopal™, a novel platform ideal for efficient focused microarray analysis. Genopal™, which consists of gel-filled fibres, is advantageous for high-quality mass production via large-scale slicing of the Genopal™ block. We prepared two arrays, infectant and autoimmunity, that provided highly reliable data in terms of repetitive scanning of the same and/or distinct microarrays. Moreover, we demonstrated that Genopal™ had sensitivity sufficient to yield signals in short hybridization times (0.5 h). Application of the autoimmunity array to blood samples allowed us to identify an expression pattern specific to Takayasu arteritis based on the Spearman rank correlation by comparing the reference profile with those of several autoimmune diseases and healthy volunteers (HVs). The comparison of these data with those obtained by other methods revealed that they exhibited similar expression profiles of many target genes. Taken together, these data demonstrate that Genopal™ is an advantageous platform for focused microarrays with regard to its low cost, rapid results and reliable quality.

Keywords: focused microarray; Takayasu arteritis; spearman; RNA diagnostics; transcriptome

Journal Article.  6430 words.  Illustrated.

Subjects: Genetics and Genomics

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