Journal Article

Identification of <i>Cis</i>-Acting Promoter Elements in Cold- and Dehydration-Induced Transcriptional Pathways in Arabidopsis, Rice, and Soybean

Kyonoshin Maruyama, Daisuke Todaka, Junya Mizoi, Takuya Yoshida, Satoshi Kidokoro, Satoko Matsukura, Hironori Takasaki, Tetsuya Sakurai, Yoshiharu Y. Yamamoto, Kyouko Yoshiwara, Mikiko Kojima, Hitoshi Sakakibara, Kazuo Shinozaki and Kazuko Yamaguchi-Shinozaki

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 19, issue 1, pages 37-49
Published in print February 2012 | ISSN: 1340-2838
Published online December 2011 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/dsr040

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The genomes of three plants, Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa), and soybean (Glycine max), have been sequenced, and their many genes and promoters have been predicted. In Arabidopsis, cis-acting promoter elements involved in cold- and dehydration-responsive gene expression have been extensively analysed; however, the characteristics of such cis-acting promoter sequences in cold- and dehydration-inducible genes of rice and soybean remain to be clarified. In this study, we performed microarray analyses using the three species, and compared characteristics of identified cold- and dehydration-inducible genes. Transcription profiles of the cold- and dehydration-responsive genes were similar among these three species, showing representative upregulated (dehydrin/LEA) and downregulated (photosynthesis-related) genes. All (46 = 4096) hexamer sequences in the promoters of the three species were investigated, revealing the frequency of conserved sequences in cold- and dehydration-inducible promoters. A core sequence of the abscisic acid-responsive element (ABRE) was the most conserved in dehydration-inducible promoters of all three species, suggesting that transcriptional regulation for dehydration-inducible genes is similar among these three species, with the ABRE-dependent transcriptional pathway. In contrast, for cold-inducible promoters, the conserved hexamer sequences were diversified among these three species, suggesting the existence of diverse transcriptional regulatory pathways for cold-inducible genes among the species.

Keywords: plant genome; cis-acting promoter elements; cold; dehydration; microarray

Journal Article.  6625 words.  Illustrated.

Subjects: Genetics and Genomics

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