Journal Article

A molecular mechanism of integrin regulation from bone cells stimulated by orthodontic forces

R. S. Carvalho, A. Bumann, C. Schwarzer, E. Scott and H. K.Yen

in The European Journal of Orthodontics

Published on behalf of European Orthodontics Society

Volume 18, issue 1, pages 227-235
Published in print January 1996 | ISSN: 0141-5387
e-ISSN: 1460-2210 | DOI: http://dx.doi.org/10.1093/ejo/18.1.227
A molecular mechanism of integrin regulation from bone cells stimulated by orthodontic forces

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SUMMARY

The purpose of this paper is to discuss a molecular mechanism in the signal transduction pathways of the regulation of integrin genes taking place in bone cells as a result of orthodontic or mechanical stimulation. Human osteosarcoma (HOS) TE-85 cells were cultured in Dulbecco's modified Eagle's medium (DMEM)/F-12 and grown to confluency in Flexercell type I dishes and orthodontic forces were applied to the cells via an intermittent strain of 3 cycles/minute using the Flexercell Strain Unit System for periods of 15 and 30 minutes, 2 and 24 hours and 3 days. Antibodies against β1 and α1 integrins were immunolocalized in strained and unstrained cultures. Total RNA was extracted and cDNA probes were used to measure at various mRNA expression of β1(1.2 kb) and αv (1.1 kb) integrins. A cDNA probe for cyclophylin (750 b) was used for controls of gene expression. Results showed that mechanical stimulation caused a reorganization of integrin distribution in comparison with non-stimulated controls. mRNA for β1 expression showed a marked increase at 30 minutes and 3 days, while mRNA levels for αv did not change with strain. The selective expression of integrins mRNA is indicative of a specific gene regulation by mechanical stimulation in the cells studied.

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Subjects: Restorative Dentistry and Orthodontics

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