Journal Article

Endothelin‐1 expression in the microvasculature of normal and 3‐hour continuously loaded rat molar periodontal ligament

Milton R. Sims

in The European Journal of Orthodontics

Published on behalf of European Orthodontics Society

Volume 23, issue 6, pages 647-662
Published in print December 2001 | ISSN: 0141-5387
Published online December 2001 | e-ISSN: 1460-2210 | DOI: https://dx.doi.org/10.1093/ejo/23.6.647
Endothelin‐1 expression in the microvasculature of normal and 3‐hour continuously loaded rat molar periodontal ligament

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Endothelin‐1 (ET‐1) distribution is described in the microvascular bed (MVB) of normal rat molar periodontal ligament (PDL). Five male Sprague–Dawley rats, aged 90 days, were anaesthetized and an external pressure of 100 ± 20 g, maintained for 3 hours, was transmitted occlusally to randomly allocated left or right molars. The controls were the contralateral molars.

Rats were perfused for 5 minutes with 5 per cent paraformaldehyde, and the mandibles post‐fixed and stored in 30 per cent sucrose. Sagittal, undemineralized, mandibular jaw sections, ∼150 µm thick, were immunolabelled with ET‐1 and alpha‐smooth muscle actin (α‐SMA) primary antibodies, and IgG/CY5 and IgG/CY3 secondary antibodies, respectively. Serial images were captured with a Bio‐Rad MRC‐1000UV confocal laser scanning microscope (CLSM).

In the control PDL, the ET‐1 immunolabelling occurred sporadically in all categories of PDL blood vessels. ET‐1 showed a punctate distribution within endothelial cells, producing longitudinal and circumferential pan‐endothelial labelling. Immunoreactivity to the ET‐1 antibody occurred at or adjacent to vessel branching sites, and affected blood vessels with and lacking α‐SMA immunolabelling.

Treatment effects on ET‐1 immunofluorescence were analysed for vascular endothelium, socket bone surface cells, cementum surface cells and PDL background in the cervical, inter‐radicular and apical regions. Significant (P < 0.05) region by treatment interactions occurred for the endothelium and bone. Cementum showed a significant (P < 0.05) region effect and a significant (P < 0.05) treatment effect. However, the region by treatment interaction was not significant. Background immunofluorescence showed significant (P < 0.05) region by treatment effects for the endothelium and bone. ET‐1 activity is the default state for normal PDL vascular endothelium.

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Subjects: Restorative Dentistry and Orthodontics

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