Journal Article

Low-energy laser irradiation facilitates the velocity of tooth movement and the expressions of matrix metalloproteinase-9, cathepsin K, and alpha(v) beta(3) integrin in rats

Masaru Yamaguchi, Masami Hayashi, Shouji Fujita, Takamasa Yoshida, Tadahiko Utsunomiya, Hirotsugu Yamamoto and Kazutaka Kasai

in The European Journal of Orthodontics

Published on behalf of European Orthodontics Society

Volume 32, issue 2, pages 131-139
Published in print April 2010 | ISSN: 0141-5387
Published online February 2010 | e-ISSN: 1460-2210 | DOI: http://dx.doi.org/10.1093/ejo/cjp078
Low-energy laser irradiation facilitates the velocity of tooth movement and the expressions of matrix metalloproteinase-9, cathepsin K, and alpha(v) beta(3) integrin in rats

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It has previously been reported that low-energy laser irradiation stimulated the velocity of tooth movement via the receptor activator of nuclear factor kappa B (RANK)/RANK ligand and the macrophage colony-stimulating factor/its receptor (c-Fms) systems. Matrix metalloproteinase (MMP)-9, cathepsin K, and alpha(v) beta(3) [α(v)β3] integrin are essential for osteoclastogenesis; therefore, the present study was designed to examine the effects of low-energy laser irradiation on the expression of MMP-9, cathepsin K, and α(v)β3 integrin during experimental tooth movement.

Fifty male, 6-week-old Wistar strain rats were used in the experiment. A total force of 10g was applied to the rat molars to induce tooth movement. A Ga-Al-As diode laser was used to irradiate the area around the moving tooth and, after 7 days, the amount of tooth movement was measured. To determine the amount of tooth movement, plaster models of the maxillae were made using a silicone impression material before (day 0) and after tooth movement (days 1, 2, 3, 4, and 7). The models were scanned using a contact-type three-dimensional (3-D) measurement apparatus. Immunohistochemical staining for MMP-9, cathepsin K, and integrin subunits of α(v)β3 was performed. Intergroup comparisons of the average values were conducted with a Mann–Whitney U-test for tooth movement and the number of tartrate-resistant acid phosphatase (TRAP), MMP-9, cathepsin K, and integrin subunits of α(v)β3-positive cells.

In the laser-irradiated group, the amount of tooth movement was significantly greater than that in the non-irradiated group at the end of the experiment (P < 0.05). Cells positively stained with TRAP, MMP-9, cathepsin K, and integrin subunits of α(v)β3 were found to be significantly increased in the irradiated group on days 2–7 compared with those in the non-irradiated group (P < 0.05).

These findings suggest that low-energy laser irradiation facilitates the velocity of tooth movement and MMP-9, cathepsin K, and integrin subunits of α(v)β3 expression in rats.

Journal Article.  4209 words.  Illustrated.

Subjects: Restorative Dentistry and Orthodontics

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