Journal Article

Cytotoxicity of orthodontic bonding adhesive resins on human oral fibroblasts

Farzaneh Ahrari, Jalil Tavakkol Afshari, Maryam Poosti and Azam Brook

in The European Journal of Orthodontics

Published on behalf of European Orthodontics Society

Volume 32, issue 6, pages 688-692
Published in print December 2010 | ISSN: 0141-5387
Published online June 2010 | e-ISSN: 1460-2210 | DOI:
Cytotoxicity of orthodontic bonding adhesive resins on human oral fibroblasts

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  • Restorative Dentistry and Orthodontics


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There is little information concerning the cytotoxic effects of no-mix and flowable adhesives used in orthodontics. The aim of the present study was to evaluate the cytotoxic effects of a no-mix (Unite), a light-cured (Tranbond XT), and a flowable (Denfil Flow) adhesives on human oral fibroblasts.

Twelve discs of each adhesive were prepared and aged for 1, 3, 5, and 7 days in Dulbecco's Modified Eagle's Medium (DMEM). Cell viability was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and the difference between the groups was tested by analysis of variance and Tukey tests (α = 0.05).

After 1 day of storage, the no-mix adhesive showed moderate cytotoxic effects (P < 0.05), while the light-cured and flowable adhesives were essentially non-cytotoxic. Ageing considerably reduced the cytotoxicity of the no-mix adhesive. On days 5 and 7 of the experiment, the cell viability of three adhesives did not differ significantly (P > 0.05), but cell viability was slightly reduced on day 7.

Moderate cytotoxic effects of no-mix adhesive on the first day of the experiment suggest that care should be taken to protect dentists and patients when these adhesives are being handled. Despite higher resin components, the flowable adhesive showed excellent biocompatibility.

Journal Article.  3295 words.  Illustrated.

Subjects: Restorative Dentistry and Orthodontics

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