Journal Article

Genome Sequence of the Mesophilic Thermotogales Bacterium <i>Mesotoga prima</i> MesG1.Ag.4.2 Reveals the Largest Thermotogales Genome To Date

Olga Zhaxybayeva, Kristen S. Swithers, Julia Foght, Anna G. Green, David Bruce, Chris Detter, Shunsheng Han, Hazuki Teshima, James Han, Tanja Woyke, Sam Pitluck, Matt Nolan, Natalia Ivanova, Amrita Pati, Miriam L. Land, Marlena Dlutek, W. Ford Doolittle, Kenneth M. Noll and Camilla L. Nesbø

in Genome Biology and Evolution

Published on behalf of Society for Molecular Biology and Evolution

Volume 4, issue 8, pages 700-708
Published in print January 2012 |
Published online July 2012 | e-ISSN: 1759-6653 | DOI:

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Here we describe the genome of Mesotoga prima MesG1.Ag4.2, the first genome of a mesophilic Thermotogales bacterium. Mesotoga prima was isolated from a polychlorinated biphenyl (PCB)-dechlorinating enrichment culture from Baltimore Harbor sediments. Its 2.97 Mb genome is considerably larger than any previously sequenced Thermotogales genomes, which range between 1.86 and 2.30 Mb. This larger size is due to both higher numbers of protein-coding genes and larger intergenic regions. In particular, the M. prima genome contains more genes for proteins involved in regulatory functions, for instance those involved in regulation of transcription. Together with its closest relative, Kosmotoga olearia, it also encodes different types of proteins involved in environmental and cell–cell interactions as compared with other Thermotogales bacteria. Amino acid composition analysis of M. prima proteins implies that this lineage has inhabited low-temperature environments for a long time. A large fraction of the M. prima genome has been acquired by lateral gene transfer (LGT): a DarkHorse analysis suggests that 766 (32%) of predicted protein-coding genes have been involved in LGT after Mesotoga diverged from the other Thermotogales lineages. A notable example of a lineage-specific LGT event is a reductive dehalogenase gene—a key enzyme in dehalorespiration, indicating M. prima may have a more active role in PCB dechlorination than was previously assumed.

Keywords: lateral gene transfer; thermotogales; mesophilic; temperature adaptation

Journal Article.  4349 words.  Illustrated.

Subjects: Bioinformatics and Computational Biology ; Evolutionary Biology ; Genetics and Genomics

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