Journal Article

Complete Genome Sequence of <i>Streptococcus dysgalactiae</i> subsp. <i>equisimilis</i> 167 Carrying Lancefield Group C Antigen and Comparative Genomics of <i>S. dysgalactiae</i> subsp. <i>equisimilis</i> Strains

Shinya Watanabe, Teruo Kirikae and Tohru Miyoshi-Akiyama

in Genome Biology and Evolution

Published on behalf of Society for Molecular Biology and Evolution

Volume 5, issue 9, pages 1644-1651
Published in print January 2013 |
Published online August 2013 | e-ISSN: 1759-6653 | DOI: http://dx.doi.org/10.1093/gbe/evt117

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Streptococcus dysgalactiae subsp. equisimilis (SDSE) is an emerging human pathogen that causes life-threatening invasive infections such as streptococcal toxic shock syndrome. Recent epidemiological studies reveal that invasive SDSE infections have been increasing in Asia, Europe, and the United States. Almost all SDSE carry Lancefield group G or C antigen. We have determined the complete genome sequence of a human group C SDSE 167 strain. A comparison of its sequence with that of four SDSE strains, three in Lancefield group G and one in Lancefield group A, showed approximately 90% coverage. Most regions showing little or no homology were located in the prophages. There was no evidence of massive rearrangement in the genome of SDSE 167. Bayesian phylogeny using entire genome sequences showed that the most recent common ancestor of the five SDSE strains appeared 446 years ago. Interestingly, we found that SDSE 167 harbors sugar metabolizing enzymes in a unique region and streptodornase in the phage region, which presumably contribute to the degradation of host tissues and the prompted covRS mutation, respectively. A comparison of these five SDSE strains, which differ in Lancefield group antigens, revealed a gene cluster presumably responsible for the synthesis of the antigenic determinant. These results may provide the basis for molecular epidemiological research of SDSE.

Keywords: Streptococcus dysgalactiae subsp. equisimilis; Lancefield group C; complete genome sequence; Bayesian phylogeny

Journal Article.  2670 words.  Illustrated.

Subjects: Bioinformatics and Computational Biology ; Evolutionary Biology ; Genetics and Genomics

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