Journal Article

RNAi screening of human glycogene orthologs in the nematode <i>Caenorhabditis elegans</i> and the construction of the <i>C. elegans</i> glycogene database

Sayaka Akiyoshi, Kazuko H Nomura, Katsufumi Dejima, Daisuke Murata, Ayako Matsuda, Nanako Kanaki, Tetsuro Takaki, Hiroyuki Mihara, Takayuki Nagaishi, Shuhei Furukawa, Keiko-Gengyo Ando, Sawako Yoshina, Shohei Mitani, Akira Togayachi, Yoshinori Suzuki, Toshihide Shikanai, Hisashi Narimatsu and Kazuya Nomura

in Glycobiology

Volume 25, issue 1, pages 8-20
Published in print January 2015 | ISSN: 0959-6658
Published online August 2014 | e-ISSN: 1460-2423 | DOI: http://dx.doi.org/10.1093/glycob/cwu080
RNAi screening of human glycogene orthologs in the nematode Caenorhabditis elegans and the construction of the C. elegans glycogene database

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In this study, we selected 181 nematode glycogenes that are orthologous to human glycogenes and examined their RNAi phenotypes. The results are deposited in the Caenorhabditis elegans Glycogene Database (CGGDB) at AIST, Tsukuba, Japan. The most prominent RNAi phenotypes observed are disruptions of cell cycle progression in germline mitosis/meiosis and in early embryonic cell mitosis. Along with the previously reported roles of chondroitin proteoglycans, glycosphingolipids and GPI-anchored proteins in cell cycle progression, we show for the first time that the inhibition of the functions of N-glycan synthesis genes (cytoplasmic alg genes) resulted in abnormal germline formation, ER stress and small body size phenotypes. The results provide additional information on the roles of glycoconjugates in the cell cycle progression mechanisms of germline and embryonic cells.

Keywords: alg genes; germline cell division; glycogene; oligosaccharyltransferase; RNAi

Journal Article.  8214 words.  Illustrated.

Subjects: Carbohydrates

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