Journal Article

The mitochondrial protein frataxin prevents nuclear damage

Gopalakrishnan Karthikeyan, L. Kevin Lewis and Michael A. Resnick

in Human Molecular Genetics

Volume 11, issue 11, pages 1351-1362
Published in print May 2002 | ISSN: 0964-6906
Published online May 2002 | e-ISSN: 1460-2083 | DOI:
The mitochondrial protein frataxin prevents nuclear damage

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The mitochondrial protein frataxin helps maintain appropriate iron levels in the mitochondria of yeast and humans. A deficiency of this protein in humans causes Friedreich's ataxia, while its complete absence in yeast (Δyfh1 mutant) results in loss of mitochondrial DNA, apparently due to radicals generated by excess iron. We found that the absence of frataxin in yeast also leads to nuclear damage, as evidenced by inducibility of a nuclear DNA damage reporter, increased chromosomal instability including recombination and mutation, and greater sensitivity to DNA-damaging agents, as well as slow growth. Addition of a human frataxin mutant did not prevent nuclear damage, although it partially complemented the Δyfh1 mutant in preventing mitochondrial DNA loss. The effects in Δyfh1 mutants result from reactive oxygen species (ROS), since (i) Δyfh1 cells produce more hydrogen peroxide, (ii) the effects are alleviated by a radical scavenger and (iii) the glutathione peroxidase gene prevents an increase in mutation rates. Thus, the frataxin protein is concluded to have a protective role for the nucleus as well as the mitochondria.

Journal Article.  7588 words.  Illustrated.

Subjects: Genetics and Genomics

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