Journal Article

Experimental analysis of the annotation of promoters in the public database

Sharon L. Coleman, Paul R. Buckland, Bastiaan Hoogendoorn, Carol Guy, Kaye Smith and Michael C. O'Donovan

in Human Molecular Genetics

Volume 11, issue 16, pages 1817-1821
Published in print August 2002 | ISSN: 0964-6906
Published online August 2002 | e-ISSN: 1460-2083 | DOI: http://dx.doi.org/10.1093/hmg/11.16.1817
Experimental analysis of the annotation of promoters in the public database

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The ability to identify and examine promoter elements is important to researchers who wish to understand how gene expression is regulated in normal and pathological states. Unfortunately, the number of human promoters that have been directly experimentally defined is small. In order to determine if promoter sequences can be identified by simply aligning mRNA and genomic sequences, we have used a reporter gene assay to assess the promoter activity of the immediate 5′ region flanking 38 mRNAs mapping to chromosome 21. For comparison, we have measured the activities of 19 sequences not thought to be promoters and 39 sequences taken from the Eukaryotic Promoter Database. Our results suggest that alignment of reference mRNAs to genomic sequence allows promoters to be identified for at least 75% of genes. These data provide the first empirical evidence that the current state of annotation of the genome is sufficient to allow molecular geneticists to correctly identify promoter sequences for most genes for which reference mRNA and genomic sequences are available.

Journal Article.  3353 words.  Illustrated.

Subjects: Genetics and Genomics

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