Journal Article

Mutational Scanning of Large Genes By Extensive Pcr Multiplexing and Two-Dimensional Electrophoresis: Application to the <i>RB1</i> Gene

Nathalie J. Van Orsouw, Daizong Li, Pieter van der Vlies, Hans Scheffer, Charis Eng, Charles H. C. M. Buys, Frederick P. Li and Jan Vijg

in Human Molecular Genetics

Volume 5, issue 6, pages 755-761
Published in print June 1996 | ISSN: 0964-6906
Published online June 1996 | e-ISSN: 1460-2083 | DOI: http://dx.doi.org/10.1093/hmg/5.6.755
Mutational Scanning of Large Genes By Extensive Pcr Multiplexing and Two-Dimensional Electrophoresis: Application to the RB1 Gene

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With the rapid increase in the number of identified human disease genes, the development of accurate and cost-efficient mutation tests has become opportune. Here we present a combination of extensive PCR multiplexing and two-dimensional (2-D) DNA electrophoresis to screen for mutations in 26 exons of the retinoblastoma (RB1) tumor suppressor gene. In 2-D electrophoresis, fragments are separated according to size and base pair sequence in non-denaturing and denaturing gradient gels, respectively. All target fragments, designed to have optimal melting characteristics, were prepared in a two-step PCR (a 6-plex long-PCR pre-amplification and a subsequent 25-plex short-PCR) followed by heteroduplexing. The mixture of PCR amplicons was then subjected to 2-D electrophoresis under a single set of experimental conditions. With this design, 35 previously identified mutations in 18 different exons were detected in 33 bilateral retinoblastoma patients. These results suggest that 2-D electrophoresis in this format provides a generally applicable, practical and fast way to diagnose with high accuracy large genes for a broad spectrum of possible disease-causing mutations.

Journal Article.  4231 words.  Illustrated.

Subjects: Genetics and Genomics

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