Journal Article

Identification of a Self-Association Region Within the <i>SCA1</i> Gene Product, Ataxin-1

Eric N. Burright, Jennifer D. Davidson, Lisa A. Duvick, Beena Koshy, Huda Y. Zoghbi and Harry T. Orr

in Human Molecular Genetics

Volume 6, issue 4, pages 513-518
Published in print April 1997 | ISSN: 0964-6906
Published online April 1997 | e-ISSN: 1460-2083 | DOI:
Identification of a Self-Association Region Within the SCA1 Gene Product, Ataxin-1

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Spinocerebellar ataxia type 1 (SCA1) is an autosomal dominant neurodegenerative disorder caused by the expansion of a polyglutamine tract within the SCA1 gene product, ataxin-1. Expansion of this tract is believed to result in a gain of function by the mutant protein, perhaps through altered self-associations or interactions with other cellular proteins. We have used the yeast two hybrid system to determine if ataxin-1 is capable of multimerization. This analysis revealed that ataxin-1 does have the ability to self-associate, however, this association does not appear to be influenced by expansion of the polyglutamine tract. Consistent with this finding, deletion analysis excluded the involvement of the polyglutamine tract in ataxin-1 self-association, and instead localized the multimerization region to amino acids 495–605 of the wild type protein. These results, while identifying an ataxin-1 self-interaction region, fail to support a proposed model of polar-zipper mediated multimerization involving the ataxin-1 polyglutamine tract.

Journal Article.  3832 words.  Illustrated.

Subjects: Genetics and Genomics

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